Senior Research Associate

Primary Responsibilities:

• Optimize editing, growth and expansion of T lymphocytes.
• Generate novel genetic edits and immuno-phenotype and functionally characterize the resulting lymphocyte populations.
• Design and implement cloning strategies to construct plasmids for the production of mRNA and protein reagents used in primary lymphocytes.
• Implement novel and established assays to assess large-scale genomic rearrangements and off-target effects of base editing technologies.
• Design and execute DNA/RNA purification and quantification assays (qPCR, ddPCR and RNA-seq).

Qualifications:

• BS/MS in cell biology, immunology, molecular biology or related discipline with 2+ years’ experience in immunology.
• Experience culturing primary T lymphocytes is required. Experience culturing iPS cells is a plus.
• Experience in flow cytometry and immuno-phenotyping, including surface and intracellular staining.
• Experience using emerging technologies such as high-throughput sequencing and droplet digital PCR.
• Strong experience in immunostaining and flow cytometry.
• Plasmid cloning experience is a big plus